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所　　　属：　東京農業大学
基幹研究：　イニシアティブ3　「地域生存基盤の再生研究」
研究課題：　産業林持続のための樹木育種

研究テーマ：　
 

1. 植物細胞壁の研究
2. 樹木の育種と森を取りもどす研究
3. セルロース合成と分解に主眼を置いた組換え樹木の野外試験

参考文献（1～2報）：　
 

• T. Hayashi: The Science and Lore of the Plant Cell Wall - Biosynthesis, Structure and Function, BrownWalker Press, Florida USA (2006).
• T. Hayashi, K. Yoshida, Y.W. Park, T. Konishi and K. Baba: Cellulose metabolism in plants, International Review of Cytology, 247, 1-34 (2005).

平成19年度　G-COE関係業績：
 

• A Alonso-Simon, P Garcia-Angulo, AE Encina, JM Alvarez, JL Acebes and T Hayashi 2007 Increase in XET activity in bean ( Phaseolus vulgaris L.) cells habituated to dichlobenil, Planta, 226: 765-771．
• N Nishikubo, T Awano, A Banasiak, V Bourquin, F Ibatullin, R Funada, H Brumer, TT Teeri, T Hayashi, B Sundberg and EJ Mellerowicz 2007 Xyloglucan Endo-transglycosylase (XET) Functions in Gelatinous Layers of Tension Wood Fibers in Poplar—A Glimpse into the Mechanism of the Balancing Act of Trees, Plant Cell Physiol, 48: 843-855.
• BR Urbanowicz, AB Bennett, E del Campillo, C Catalá, T Hayashi, B Henrissat, H Höfte, SJ McQueen-Mason, SE Patterson, O Shoseyov, TT Teeri, and JKC Rose 2007 Structural organization and a standardized nomenclature for plant endo-1,4--glucanases (cellulases) of glycosyl hydrolase family 9. Plant Physiology, 144: 1693-1696．
• T Hayashi, YW Park, A Isogai and T Nomura 2008 Cross-linking of plant cell walls with dehydrated fructose by smoke-heat treatment. Journal of Wood Science, 54: 90-93.
• 林　隆久 産業利用をめざした遺伝子組換えポプラの野外試験がはじまる、科学技術動向月報(2007)．
• 林　隆久 民族の問題、サステナ、 2、 46-47 (2007).
• 林　隆久 遺伝子組換えポプラ、サステナ、3、42-43 (2007)．
• 林　隆久 セルラーゼ、 サステナ、4、 36-37 (2007)．
• 林　隆久 自由研究、サステナ、5、34-35 (2007)．
• 林　隆久 知の融合のかたち、サステナ、６、40-41 (2007)．
• 林　隆久 「(サイエンス)最新技術を駆使した森林復元」、『日本経済新聞』 2008年3月30日．
• 林　隆久 「（インタビュー）植物で未来をつくる」、松永和紀著、2008年3月30日、化学同人出版．
• 林　隆久、馬場啓一　糖化用植物および植物由来原料の調製方法、特願2008-077110、 2007

平成19年度 研究成果発表（口頭発表）：
 

• T. Hayashi, M. Takeuchi, Y.W. Park, T. Kaku, M. Yoshida, T. Awano, R. Kaida, K. Baba: Xyloglucan creates tensile stress in the secondary wall. XI Cell Wall Meeting 15th August 2007.
• 林　隆久 Current situation of researches on genetically modified trees in Japan、フォーラム: 少資源国日本のバイオマス研究、日本分子生物学会生化学会合同大会、パシフィコ横浜、2007年12月14日
• 林　隆久、馬場啓一　さまざまな細胞壁分解酵素を発現する組換えポプラ、理研シンポジウム、理研横浜、2008年2月18日
• T. Hayashi　Tropical trees in Southeast Asia - A reformation scenario from deforestation, Plant Science and Genetics seminar, the Hebrew University of Jerusalem, 18th December 2007.

平成19年度 研究成果発表事前通知書（論文発表）

• 査読付論文：Plant Physiology
• 査読付論文：Plant Polysaccharides（Blackwell Publishing）
• 査読付論文：Journal of wood Science

平成20年度　G-COE関係業績：
 

• T Hayashi, YW Park, A Isogai and T Nomura, Cross-linking of plant cell walls with dehydrated fructose by smoke-heat treatment. J Wood Sci, 54: 90-93 (2008)
• T Taniguchi, Y Ohmiya, M, Kurita, M Tsubomura, T Kondo, YW Park, K Baba, T Hayashi, Biosafety assessment of transgenic poplars overexpressing xyloglucanase (AaXEG2) prior to field trials, J Wood Sci, 54:408-413 (2008)
• R Kaida, T Hayashi, TS Kaneko, Purple acid phosphatase in the walls of tobacco cells, Phytochem,　69: 2546-2551 (2008)
• T Takabe T, A Uchida A, F Shinagawa, Y Terada, H Kajita, Y Tanaka, T Takabe, T Hayashi, T Kawai, T Takabe, Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants, Plant Growth Reg, 56: 265-273 (2008)
• T Takabe T, A Uchida A, F Shinagawa, Y Terada, H Kajita, Y Tanaka, T Takabe, T Hayashi, T Kawai, T Takabe, Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants, Plant Growth Reg, 56: 265-273 (2008)
• S Hartati, E Sudarmonowati E, YW Park YW, T Kaku, R Kaida, K Baba, T Hayashi, Overexpression of poplar cellulase accelerates growth and disturbs the closing　movements of leaves in sengon, Plant Physiol, 147: 552-561 (2008)
• H Ikegaya, T Hayashi, T Kaku, K Twata, S Sonobe, T Shimmen: Presence of xyloglucan-like　polysaccharide in Spirogyra and possible involvement in cell-cell attachment, Phycological Res, 56: 216-222 (2008)
• T Hayashi, R Kaida, T Kaku, K Baba, Enhancement of saccharification by overexpression of various　endoglycanase in poplar, GEO Giacaglia ed, pp 145-149, UNINDU, Taubate, Brasil (2008)
• 林　隆久、加来友美、海田るみ、馬場啓一：植物でセルラーゼを過剰発現させる, Cellulose Communication, 15, 148-152 (2008).
• 林　隆久 編集: 森をとりもどすために、海青社、大津、滋賀 （2008）
• K Ozaki, A Uchida, T Takabe, F Shinagawa, Y Tanaka, T Takabe, T Hayashi, T Hattori, AK. Raid,　T Takabe, Enrichment of sugar content in melon fruits by hydrogen peroxide treatment, J Plant　Physiol, 166: 569-578 (2009)

平成20年度 研究成果発表（口頭発表）：
 

• T Hayashi: Enhancement of saccharification by overexpression of various endoglycanase in poplar,　International Symposium on Clean Energy for the World, Ethanol Biodiesel and Nayural Gas,　Ubatuba, Brasil, December 7-10, 2008 (招待講演)
• T Hayashi: Transgenic poplars, Plant feedstock seminar, Joint BioEnergy Institute, Emeryville, USA,　15th December (2008)
• T Hayashi: Transgenic poplars, Plant biology seminar, Sao Paulo University, Sao Paulo, Brasil, 12th　December (2008)
• T Hayashi: Proposed strategies and prospects for Riau biosphere reserve, Workshop on Riau　biosphere researve, Pekanbaru, Indonesia, February 20, 2009
• T Hayashi: Loosening xyloglucan accelerates the enzymatic hydrolysis of cellulose in wood, Nissan International Tree Biology, Tokyo, February 26, 2009 (招待講演)

平成21年度　G-COE関係業績：

• Hayashi T., Kaida R., Kaku T., Baba K. (2010) Loosening xyloglucan prevents tensile stress in tree stem bending but accelerates the enzymatic degradation of cellulose. Russian J. Plant Physiol. 57, 316-320.
• Kaida R., Kaku T., Baba K., Oyadomari M., Watanabe T., Nishida K., Kanaya T., Shani Z., Shoseyov O., Hayashi T. (2009) Loosening xyloglucan accelerates the enzymatic degradation of cellulose in wood. Mol. Plant 2, 904-909.
• Kaida R., Kaku T., Baba K., Hayashi T. (2009) Enzymatic saccharification and ethanol production of Acacia mangium and Paraserianthes falcataria wood, and oil palm trunk. J. Wood Sci. 55, 381-386.
• Tomomi Kaku, Satoshi Serada, Kei’ichi Baba, Fumio Tanaka, Takahisa Hayashi (2009) Proteomic analysis of the G-layer in poplar tension wood, J Wood Sci 55:250–257.
• Kaida R., Kaku T., Baba K., Hartati S., Sudarmonowati E., Hayashi T. (2009) Enhancement of saccharification by overexpression of poplar cellulase in sengon. J. Wood Sci. 55,435-440.
• Kaida R., Satoh Y., Bulone V., Yamada Y., Kaku T., Hayashi T., Kaneko T. (2009) Activation of -glucan synthases by wall-bound purple acid phosphatase in tobacco cells. Plant Physiol. 150, 1822-1830.
• Baba K., Park W.P., Kaku T., Kaida R., Takeuchi M., Yoshida M., Hosoo Y., Ojio Y., Okuyama T., Taniguchi T., Ohmiya Y., Kondo T., Shani J., Shoseyov O., Awano T., Serada S, Norioka N., Norioka S., Hayashi T. (2009) Xyloglucan for generating tensile stress to bend tree stem. Mol. Plant 2, 893-903.
• Hayashi T., Kaida R., Mistuda N., Ohme-Takagi M., Nishikubo N., Kidou S., Yoshida K. (2009) Enhancing primary raw materials for biofuels. In Biomass to Biofuels, Wiley, pp 459-489.
• 林　隆久：セルロースの生合成、木質の化学、日本木材学会編、p. 5-12、文永堂出版2010年5月11日
• 林　隆久: バイオエタノール, サステナ, 11, 56-57 (2009).
• 林　隆久: スマトラ島エコツアー, サステナ, 13, 44-45 (2009).
• 林　隆久: 食べる幸せ, サステナ, 14, 58-59 (2010).
• 林　隆久: 食べる楽しみ, サステナ, 特別号, 56-57 (2010).

平成21年度 研究成果発表（口頭発表）：

• T Hayashi: Bioethanol Revolution, 2rd Humanosphere Science school, Cibinong, Indonesia, March 26-27 (2009)
• T Hayashi: Case study on bioethanol production in Riau, 3rd Humanosphere Science school, Pekanbaru, Indonesia, August 4-5 (2009)
• T Hayashi: Transgenic trees overexpressing AaXEG, Plant Science and Genetic Seminar, the Hebrew University of Jerusalem, November 25 (2009)
• T Hayashi: Our strategy on Riau Biosphere Reserve, International Workshop on Sustainable Forest Tree Plantation in the Giam Siak-Bukit Batu Biosphere Reserve of Indonesia, Kyoto, Japan, February 4 (2010)